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Vol. 61 No. 2 (2025)
DOI: 10.4415/ANN_25_02_08
Original Articles
2025-06-06
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A preliminary study of the effects of transformations induced by gammaray treatment on the detection of Acheta domesticus and Tenebrio molitor allergenic proteins by enzyme-linked immunosorbent assay (ELISA) techniques

Authors

Augusto Alberto Pastorelli , Matteo Rosetti , Silvia Di Giacomo , Emanuela Bortolin , Stefania Morelli , Antonella Semeraro , Concetta Boniglia , Paolo Stacchini , Raffaella Gargiulo

Abstract

Introduction. With the global population projected to reach approximately 9 billion by 2050, there’s a growing need to explore alternative food sources. Insects have emerged as a potential solution to meet food demand, offering a substitute for conventional livestock. However, a primary safety concern surrounding these novel foods is their allergenic potential, especially given the absence of standardized testing methodologies. To
mitigate this risk, food irradiation has been explored as a method to reduce allergenicity in insects intended for human and animal consumption.
Material and methods. This study utilized an enzyme-linked immunosorbent assay to determine the allergenic proteins in specific insect types after irradiation treatment.
Results and discussion. Significant differences in detectable protein levels were observed between Tenebrio molitor and Acheta domesticus samples, but no significant differences in protein content were found between food and feed samples of both species under identical irradiation conditions. Further research is required to ensure the protocol’s suitability for more complex food matrices.

Keywords: edible insects, food contamination, food hypersensitivity, enzyme-linked immunosorbent assay (ELISA), food irradiation

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Authors

Augusto Alberto Pastorelli

Matteo Rosetti - Dipartimento di Sicurezza Alimentare, Nutrizione e Sanità Pubblica Veterinaria, Istituto Superiore di Sanità, Rome, Italy

Silvia Di Giacomo - Dipartimento di Sicurezza Alimentare, Nutrizione e Sanità Pubblica Veterinaria, Istituto Superiore di Sanità, Rome, Italy

Emanuela Bortolin - Servizio Grandi Strumentazioni e Core Facilities, Istituto Superiore di Sanità, Rome, Italy

Stefania Morelli - Dipartimento di Sicurezza Alimentare, Nutrizione e Sanità Pubblica Veterinaria, Istituto Superiore di Sanità, Rome, Italy

Antonella Semeraro - Dipartimento di Sicurezza Alimentare, Nutrizione e Sanità Pubblica Veterinaria, Istituto Superiore di Sanità, Rome, Italy

Concetta Boniglia - Dipartimento di Sicurezza Alimentare, Nutrizione e Sanità Pubblica Veterinaria, Istituto Superiore di Sanità, Rome, Italy

Paolo Stacchini - Dipartimento di Sicurezza Alimentare, Nutrizione e Sanità Pubblica Veterinaria, Istituto Superiore di Sanità, Rome, Italy

Raffaella Gargiulo - Dipartimento di Sicurezza Alimentare, Nutrizione e Sanità Pubblica Veterinaria, Istituto Superiore di Sanità, Rome, Italy

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Copyright

Copyright (c) 2025 Istituto Superiore di Sanità

How to Cite
Pastorelli, A. A., Rosetti, M., Di Giacomo, S., Bortolin, E., Morelli, S., Semeraro, A., Boniglia, C., Stacchini, P., & Gargiulo, R. (2025). A preliminary study of the effects of transformations induced by gammaray treatment on the detection of Acheta domesticus and Tenebrio molitor allergenic proteins by enzyme-linked immunosorbent assay (ELISA) techniques. Annali dell’Istituto Superiore Di Sanità, 61(2), 149–156. https://doi.org/10.4415/ANN_25_02_08
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