Molecular characterization of Neisseria gonorrhoeae on non-cultured specimens from multiple anatomic sites

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Abstract

Introduction. The aim was to characterize Neisseria gonorrheae collected from multiple sites in the same patient without a cultured strain. The use of N. gonorrhoeae multiantigen sequence typing (NG-MAST) together with the gene sequence analysis of antimicrobial resistance (AMR) target genes permit to depict these samples.

Materials and methods. Seventeen genital and extra-genital samples from eight patients (7 were men who have sex with men, MSM, and 1 women who have sex with men, WSM) with gonorrhoea symptoms were analyzed.

For 7, of the 8 patients, conventional culture method has been used to identify gonorrhoea. All the samples were tested with the rapid molecular method CEPHEID. Amplification and sequencing of porB and tbpB, to identify the Sequence Type (ST) by NG-MAST, and penA, mtrR, porB1b, ponA genes were also performed.

Antimicrobial susceptibility by Etest, for the available culture positive samples, was carried out.

Results. For 7 patients it was obtained the ST (Sequence Type) and for 6 the complete sequence analysis of the antimicrobial resistance target genes. For the majority of them, samples collected from multiple sites confirm the presence of the same gonorrhoea strain. In particular, for 5 patients the same STs and changes in the AMR target genes were identified.

Conclusion. Molecular characterization on non-cultured or culture negative specimens for gonorrhoea permit to predict the presence of the same strain in the patients with infection in multiple anatomic sites and the genetic antimicrobial susceptibility pattern .

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Authors

Anna Carannante - Dipartimento di Malattie Infettive, Istituto Superiore di Sanità, Rome, Italy

Valeria Ghisetti - Dipartimento di Malattie Infettive, Laboratorio di Microbiologie e Virologia, Ospedale Amedeo di Savoia, Turin, Italy

Ivano Dal Conte - Dipartimento di Malattie Infettive, Ambulatorio IST, Ospedale Amedeo di Savoia, Turin, Italy

Gabriella Gregori - Dipartimento di Malattie Infettive, Laboratorio di Microbiologie e Virologia, Ospedale Amedeo di Savoia, Turin, Italy

Maria Laura Stella - Dipartimento di Malattie Infettive, Università di Torino, Turin, Italy

Paola Vacca - Dipartimento di Malattie Infettive, Istituto Superiore di Sanità, Rome, Italy

Simonetta Del Re - Dipartimento di Malattie Infettive, Laboratorio di Microbiologie e Virologia, Ospedale Amedeo di Savoia, Turin, Italy

Paola Stefanelli - Dipartimento di Malattie Infettive, Istituto Superiore di Sanità, Rome, Italy

How to Cite
Carannante, A., Ghisetti, V., Dal Conte, I., Gregori, G., Stella, M. L., Vacca, P., Del Re, S., & Stefanelli, P. (2017). Molecular characterization of Neisseria gonorrhoeae on non-cultured specimens from multiple anatomic sites. Annali dell’Istituto Superiore Di Sanità, 53(3), 213-217. Retrieved from https://annali.iss.it/index.php/anna/article/view/531
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